CRISPR ADVANCED CERTIFICATION COURSE – 25th May 2018 Batch

79 STUDENTS ENROLLED

Course Module:

DAY 1Revision to basics and Somatic/ Germ line modification
DAY 2Methods for mutation detection and cell manipulation using the most current technologies.
DAY 3gRNA and PAM CRISPR locus
DAY 4CAS9 and CPF1 and modern variations (CAS-FISH) – comparison
DAY 5CRISPR interference – CRISPRi and CRISPR a
DAY 6Repair – HDR, NHDR (Homology-directed repair)
DAY 7Selection of appropriate vector system (using gRNA)
DAY 8Generation of knock-in knock out cells, disease models
DAY 9Application in induced pluripotent stem cells
DAY 10Application in plant breeding and horticulture
DAY 11Application of CRISPR in genetic disorders and Biomedicine
DAY 12Co-evolution – Reverse Transcriptase associated with CRISPR CAS system
DAY 13CRISPR on-target and off target analysis
DAY 14Summary, recognitions, patents, limitations and future aspects

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